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X299-E GAMING - Don't detect second GPU anymore

PsY4Meuh
Level 7
Today I noticed that my second graphics card is no longer detected.
I tried to swap the two graphics cards but the problem persists: the one in the slot PCIEX16/X8_2 is never detected neither in the BIOS, nor in Windows!
I checked the power supplies, even a Clear CMOS does not change anything!
If I remove the first card, the PC does not boot at all, and I have no signal on the GPU

Config :
- Asus ROG Strix X299-E GAMING - Bios 0802
- Asus ROG Strix GTX1080TI OC x2 SLI
- G.Skill Trident Z 8x8GB
- Samsung 960 EVO 500GB
- Corsair HX1000i

Any advice ? Or should I just contact Asus support ?
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