At any time during this did you try and turn the 2D back on ?
What were the clock setting when you turned the 2D off ?
I would reset all the GPU Tweak settings with the old card in place then shut the system down then switch cards and put the Matrix back in with the GPU Tweak still at default settings with 2D back on and you should get the signal back !
edit: you can also try swapping the cards with a older version of GPU Tweak in place.
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