I'll share some experience I have had with this stuff (but I've never build a custom WC loop).
1. I have fried MOBO fan headers before (on my Rampage 3 extreme). Plugging in 2 x high amp fans via a Y-cable to a single 3 or 4 pin mobo header can torch it. Thankfully it didnt wreck anything else on the board.
2. I have fried the fan control "part" of an H80i as well. The h80i (like the 100i) has 2 x plugs in the CPU block for fans. Well, as far as I can tell, they are meant for 4 fans max, 2 on each. One time I wrecked it by plugg in 4 x fans via Y-cables into a single channel on the H80i. That ruined that too.
3. Asus Fan Xpert isn't perfect. Sometimes my PC starts with the fans going 100% and I have to manually click the "silent" profile and then re-load my custom one. Happens a lot actually. I did the fan test from within windows. Might try it from inside BIOS, didnt even realize there was a fan calibration option in there until your post.
A pic because Im bored:
*CPU: Intel 10980XE @ 5.0 ghz (by Core usage) w/ EK monoblock
*Mobo: Asus Rampage VIE
*RAM: 64GB DDR4 3000 G.Skill TridentZ
*Graphics: Gigabite 3090 Waterforce
*Monitor: Dell Alienware AW3418DW @ 120hz
*Storage OS: Samsung SM970 Pro (2TB) Windows 10
*Storage Games Internal: 4TB 850 EVO RAID0
*Storage Extermal: 48TB Raid0 (External USB 3.1 Box)
*Case/PSU: Thermaltake V71 TG/RGB + 3 Rads (120mm, 360mm, 420mm) + Corsair AX1200i PSU